It appeared that Hefner's wife Crystal Harris was not invited to the meal.
It is not clear whether 31-year-old wife Crystal was also by his side when he died.
He was pictured again on Father's Day this year in the Playboy Mansion in an intimate photo shared online by his son Cooper.
This is believed to be the last picture of Hefner which is in circulation.
He married Conrad the same year she became Playmate of the Year.
Their son, Cooper, now runs Playboy Hefner was born on April 9, 1926 ,in Chicago, Illinois, and went on to become a millionaire after founding the influential men's magazine in 1953. #RIPHef.' Hefner's death was confirmed in a statement from Playboy Enterprises that said he 'passed away today [Wednesday] from natural causes at his home, The Playboy Mansion, surrounded by loved ones'.